Imbibitional redox and hormonal priming revealed regulation of oxidative window as a key factor for progression of germination of indica rice cultivars.

In the present investigation we have manipulated seeds of two indica rice cultivars, differing in sensitivity towards salinity stress (Oryza sativa L. cv. IR29 and Pokkali), with different combination of germination influencing hormones and redox modulating agents [500 µM Gibberellic acid (GA) + 20 mM H(2)O(2), 500 µM GA + 100 µM Diphenyleneiodonium chloride (DPI), 500 µM GA + 500 µM N,N-dimethylthiourea (DMTU), 30 µM Triadimefon (TDM) + 100 µM DPI, 30 µM TDM + 500 µM DMTU] during early imbibition for exploring significance of regulation of oxidative window during germination. Reactive oxygen species (ROS)-antioxidant (AOX) interaction dynamics, assessed through redox metabolic fingerprints revealed significant changes in oxidative window of germinating tissue under redox and hormonal priming. GA (500 µM) + H(2)O(2) (20 mM) priming formed favorable redox cue and opened the oxidative window for germination, whereas GA (500 µM) + DPI (100 µM), GA (500 µM) + DMTU (500 µM) and TDM (30 µM) + DPI (100 µM) combination failed to generate redox cue for opening the oxidative window at metabolic interface. Assessment of transcript abundance of genes of enzymes of central redox hub (RBOH-SOD-ASC-GSH/CAT pathway) further confirmed the transcriptional reprogramming of genes (Osrboh, OsSodCc2, OsCatA, OsAPx2, OsGRase) necessary for antioxidant-coupled origin of redox cue for germination. Assessment of pool of gibberellic acid, abscisic acid and jasmonic acid revealed a close connection between the hormonal homeostasis and internal redox cue. Role of oxidative window generated during metabolic reactivation phase for successful progression of germination is suggested. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12298-023-01303-x.