Three-dimensional cerebral vasculature topological parameter extraction of transgenic zebrafish embryos with a filling-enhancement deep learning network.

Quantitative analysis of zebrafish cerebral vasculature is essential for the study of vascular development and disease. We developed a method to accurately extract the cerebral vasculature topological parameters of transgenic zebrafish embryos. The intermittent and hollow vascular structures of transgenic zebrafish embryos, obtained from 3D light-sheet imaging, were transformed into continuous solid structures with a filling-enhancement deep learning network. The enhancement enables the extraction of 8 vascular topological parameters accurately. Quantitation of the zebrafish cerebral vasculature vessels with the topological parameters show a developmental pattern transition from 2.5 to 5.5 dpf.