Abstract
We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions.
Keywords:
(060.5295) Photonic crystal fibers; (140.3510) Lasers, fiber; (170.3880) Medical and biological imaging; (180.4315) Nonlinear microscopy; (300.6230) Spectroscopy, coherent anti-Stokes Raman scattering; (300.6430) Spectroscopy, photothermal; (300.6450) Spectroscopy, Raman.