Locusts adopt IP(3) as a second messenger for olfactory signal transduction.

Insects, unlike vertebrates, use heteromeric complexes of odorant receptors and co-receptors for olfactory signal transduction. However, the secondary messengers involved in this process are largely unknown. Here, we use the olfactory signal transduction of the aggregation pheromone 4-vinylanisole (4VA) as a model to address this question. When locusts detect 4VA, the pheromone is transported by OBP10 and OBP13 to the OR35-Orco receptor complex, thereby activating downstream pathways in the antenna. A pivotal downstream molecule, the lipid-binding protein Clvs2, facilitates phosphatidylinositol 4,5-bisphosphate transportation across the cytolemma, providing more substrates for inositol trisphosphate (IP(3)) production. PLCe1, a biosynthetic enzyme, boosts IP(3) levels in the antennal lobe of the brain. IP(3) is responsible for converting chemical signals from the antenna into neural signals, confirming IP(3) as a secondary messenger in olfaction perception instead of GPCR in locusts. These findings elucidate the universal function of IP(3) in olfactory signal perception, shedding light on the key nodes of insect olfactory signal transduction.