Generation of a human induced pluripotent stem cell (iPSC) line (JUCTCi011-A) from skin fibroblasts of a healthy Jordanian male subject

从健康约旦男性受试者的皮肤成纤维细胞中生成人类诱导性多能干细胞 (iPSC) 系 (JUCTCi011-A)

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作者:Nidaa A Ababneh, Ban Al-Kurdi, Dema Ali, Raghda Barham, Nour Sharar, Mairvat M Mrahleh, Bareqa Salah, Abdalla Awidi

Abstract

Human induced pluripotent stem cell line (JUCTCi011-A) was generated from skin fibroblasts obtained from a 34-year-old healthy male subject from Jordan. The generated iPSCs showed typical embryonic-like characteristics. They retained their normal karyotype similar to their parental dermal fibroblast cells, expressed pluripotency markers and showed a differentiation potential into three germ layers as demonstrated by immunostaining and flow cytometry. This generated cell line can be used in disease modeling studies, to serve as a healthy control line and to help in developing novel therapeutic strategies for patients with hereditary neuromuscular diseases.

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