Abstract
Extracting nutraceuticals with high value from bagasse, filter mud, and sugarcane leaves discarded as sugar mill by-products, is crucial for the development of a sustainable bio-economy. These by-products are important sources of policosanols and phytosterols, which have a cholesterol-lowering effect. This research focused on using a promising green technology, subcritical liquefied dimethyl ether extraction, with a low pressure of 0.8 MPa, to extract policosanols and phytosterols and on application of pretreatments to increase their contents. For direct extraction by subcritical liquefied dimethyl ether without sample pretreatment, the highest extraction yield (7.4%) and policosanol content were found in sugarcane leaves at 2888 mg/100 g, while the highest and lowest phytosterol contents were found in filter mud at 20,878.75 mg/100 g and sugarcane leaves at 10,147.75 mg/100 g, respectively. Pretreatment of filter mud by ultrasonication in hexane solution together with transesterification before the second subcritical liquefied dimethyl ether extraction successfully increased the policosanol content, with an extract purity of 60%, but failed to increase the phytosterol content.