Abstract
Trichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, the most prevalent non-viral sexually transmitted infection, with significant impact on public health and economy. Here, we present a chromosome-level genome of T. vaginalis strain IR-78 (ATCC 50138), assembled using short and long-read sequencing technologies as well as chromatin conformation capture sequencing (Hi-C). The assembled genome is 173.3 Mb in size, with an N50 scaffold length of 26.4 Mb. The assembly is anchored in six super-scaffolds, corresponding to the six chromosomes of T. vaginalis, covering 93.5% of the genome. A total of 43,326 protein-coding genes were predicted, of which 95.4% were annotated using multiple databases, including Gene Ontology, eggNOG, and KEGG. Among these, 16,656 genes encoded hypothetical proteins. This high-quality genome assembly provides an additional resource for ongoing research on T. vaginalis pathogenesis, drug resistance, and host-parasite interactions. It furthermore enables comparative genomic studies to assess genomic variety across different Trichomonas species.