Genome sequence of Coxiella burnetii strain Namibia

伯氏柯克斯体纳米比亚菌株基因组序列

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作者:Mathias C Walter #, Caroline Öhrman #, Kerstin Myrtennäs, Andreas Sjödin, Mona Byström, Pär Larsson, Anna Macellaro, Mats Forsman #, Dimitrios Frangoulidis #

Abstract

We present the whole genome sequence and annotation of the Coxiella burnetii strain Namibia. This strain was isolated from an aborting goat in 1991 in Windhoek, Namibia. The plasmid type QpRS was confirmed in our work. Further genomic typing placed the strain into a unique genomic group. The genome sequence is 2,101,438 bp long and contains 1,979 protein-coding and 51 RNA genes, including one rRNA operon. To overcome the poor yield from cell culture systems, an additional DNA enrichment with whole genome amplification (WGA) methods was applied. We describe a bioinformatics pipeline for improved genome assembly including several filters with a special focus on WGA characteristics.

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