Abstract
During a survey of saprobic fungi, fresh specimens were collected from decaying wood in terrestrial habitats in Guizhou and Hainan provinces, southern China. Two novel species, Helicosporium qixianlingense and H. tongrenense, are introduced based on phylogenetic analyses of a combined dataset (ITS, LSU, tef1-α, and rpb2) and morphological evidence. Comprehensive descriptions, illustrations, notes, and phylogenetic analyses supporting the taxonomic placement of these new taxa are provided. These findings are significant for exploring the species diversity of Helicosporium in southern China.