Abstract
During a survey of ascomycetes in Yunnan Province, China, two new wood-inhabiting fungi were collected and identified. These novel species are described based on morphological characteristics and phylogenetic analyses of the ITS, LSU, rpb2, and tef1 loci. The newly identified species are designated as Amphisphaeria zhaotongensis and Pallidoperidium yunnanense, belonging to the families Amphisphaeriaceae and Pallidoperidiaceae, respectively. These species are confirmed as distinct from their close relatives through phylogenetic analyses and morpho-anatomical comparison. The study provides detailed morphological descriptions, illustrative representations, and a phylogenetic tree, all of which contribute to the taxonomic positioning of these novel species.