Abstract
In recent years, there has been certain progress in the research on fungal diversity in Yunnan Province, China, particularly in aquatic habitats. This study introduces two new species, Ellisembia yuxiense and Sporidesmium ailaoshanense, as well as a new host record of Sporidesmium tropicale on Pinus yunnanensis from freshwater habitats in Yunnan Province. All taxa were identified by integrating morphological traits with phylogenetic analysis of combined LSU, ITS, and rpb2 DNA sequences. Comprehensive morpho-anatomical descriptions and detailed illustrations are provided to elucidate the characteristics of each taxon.