Abstract
Members of Curvularia are of widespread clinical and agricultural importance, but species identification remains challenging due to morphological similarities and environmental variation. In this study, nine Curvularia strains were isolated from diseased leaves of Nelumbonucifera and Nicotianatabacum in Guangxi and Guizhou provinces, China. Morphological characteristics and phylogenetic analyses based on ITS, GAPDH, and tef1-α sequences revealed two novel species of Curvularia s. str., described here as C.nuciferae sp. nov. and C.tabaci sp. nov. This study enhances understanding of Curvularia taxonomy and expands the known species diversity.