Abstract
Phyllosticta (Phyllostictaceae, Botryosphaeriales) includes plant pathogens, endophytes and saprobes, occurring on various hosts worldwide. During the present study, isolates associated with leaf spots were obtained from the hosts Quercusaliena and Viburnumodoratissimum, and identified based on morphological features and phylogenetic inference from the analyses of five loci (ITS, LSU, tef1, act and gapdh). Results supported the introduction of two novel species, namely Phyllostictaanhuiensis and P.guangdongensis. Phylogenetically, P.anhuiensis and P.guangdongensis formed two well-separated lineages in the P.concentrica and P.capitalensis species complexes, distinguishing from all presently accepted species in this genus by DNA sequence data. Morphologically, P.anhuiensis and P.guangdongensis have the typical structure of the genus Phyllosticta, and differed from their closely related species by the length of the conidial appendage.