Abstract
Calycina (Pezizellaceae, Helotiales) is a heterogeneous group, and the taxonomic classification is frequently revised due to unclear phylogenetic relationships. To date, a total of 35 species in this genus have been documented, with six recorded in China, including four species that are endemic to the country. Calycina species are reported in their sexual and asexual morphs, and the phylogenetic placements of several species remain unresolved due to the lack of molecular data. During a study on the fungal diversity of southwestern China, six specimens were collected and analyzed using both morphological characterizations and multi-gene phylogenetic analyses (LSU and ITS). The results revealed two new species, C. botanica and C. xishuangbanica, and the first sexual report of C. brevipes, with detailed morphological descriptions and phylogenetic trees.