Perspective on the use of scraping cytology for jawbone destructive lesions, entamoeba gingivalis, and actinomyces co-infection: a retrospective analysis

刮取细胞学检查在颌骨破坏性病变、牙龈内阿米巴和放线菌合并感染诊断中的应用:一项回顾性分析

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Abstract

OBJECTIVES: Microbial infections can influence destructive jawbone lesions, yet the role of Entamoeba gingivalis (E. gingivalis) in these lesions is poorly understood, particularly when co-infected with Actinomyces. This study introduces a novel clinicopathological perspective on jawbone destruction linked to E. gingivalis and Actinomyces co-infection, aiming to improve diagnosis and treatment. In this study, we tried to evaluate minimally invasive methods for improving diagnosis and treatment for jawbone destruction, possibly linked to E. gingivalis and Actinomyces co-infection. We analyzed 11 cases of jawbone destructive lesions observed in patients (ages 42 to 83, both female and male) who visited Tohoku University Hospital from January 2015 to December 2021, in which scraping cytology detected E. gingivalis and Actinomyces. MATERIALS AND METHODS: We thoroughly assessed clinical symptoms, imaging findings, and treatment outcomes, including the duration of antimicrobial therapy, to identify distinctive patterns associated with this co-infection. RESULTS: On imaging, patients exhibited significant mandibular resorption and sclerosis, despite the absence of typical symptoms of osteomyelitis or actinomycosis. Notably, E. gingivalis alone was linked to faster wound healing and shorter antimicrobial therapy durations compared to cases with concurrent Actinomyces infection (p = 0.011). The use of minimally invasive procedures, focusing on removing necrotic bone and loose teeth, led to effective healing and rapid recovery across all cases, offering promising prospects for enhanced treatment outcomes. CONCLUSION: This study underscores the value of scraping cytology for early identification of E. gingivalis and Actinomyces in jawbone lesions. Early detection and targeted intervention can prevent disease progression, enhance patient outcomes, and optimize antimicrobial use. CLINICAL RELEVANCE: These findings highlight the need for increased awareness of microbial co-infections in jaw osteomyelitis and support a new clinicopathological approach to diagnosis and management.

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