Nanobiotic Formulations utilizing Quinoline-based-Triazole functionalized Carbon Quantum Dots via Click Chemistry for Combatting Clinical-Resistant Bacterial Pathogens

利用点击化学法制备喹啉基三唑功能化碳量子点纳米生物制剂,用于对抗临床耐药细菌病原体

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Abstract

Therapeutic options for preventing the trajectory of multi-drug resistance bacterial pathogens could rely on the effort to design a novel technique to develop a potent antimicrobial agent to counter the key issue. To curb the current outbreak, we synthesized first generation of antimicrobial amine-modified carbon quantum dots, CQDs-NH(2) as carbon precursors followed by hydrothermal carbonization of ethylenediamine/citric acid, and postmodified with propargyl alcohol (CQDs-1) and quinoline derivative; 8-hydroxy quinoline (CQDs-2) through Cu(I)-catalyzed azide-alkyne cycloaddition. The novel clicked 1,2,3-triazole functionalized CQDs-NH(2) templates, were evaluated against standard Gram-positive; Staphylococcus aureus (S. aureus), and Gram-negative; Escherichia coli (E. coli), MRSA, along with clinical-resistant diabetic foot PUS swab isolated bacterial pathogens by 96-well method as well as agar-well diffusion method, to unleased the potential antibacterial activity. 1,2,3-triazole functionalized CQDs-NH(2) template showed enhanced antibacterial activity against distinct bacterial strains, with minimum inhibitory concentration for standard bacteria, MRSA-bacteria, and clinical resistant bacterial pathogens in the range of 0.25-8, 64-128, and 128-256 μg mL(-1) respectively. This nanobiotic template displays good potential through the hybridization of 1,2,3-triazole with antibacterial pharmacophores CQDs-NH(2) and quinoline, to overcome drug resistance, reduce toxicity, and improve pharmacokinetic profiles. The findings of this study might have a favorable impact on antibiotic pharmacodynamics and, as a result, nanobiotic dosing regimens as well as clinical outcomes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-024-01266-x.

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