Abstract
In the present study, gold nanoparticles (AuNPs) synthesis was carried out by using a rare bacteriophage which is morphologically similar to 7-11 phages of the C3 morphotype of tailed phage belonging to Podoviridae family as green route. Effect of various physiological parameters like pH, temperature and concentration of gold chloride salt on AuNPs synthesis was studied. The reaction mixtures have shown vivid colours at various physiological parameters. Phage inspired AuNPs were further characterized by using different techniques such as UV-Vis spectrophotometry, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD) and dynamic light scattering (DLS). DLS study revealed synthesis of various sizes of AuNPs in the range of 20-100 nm. SEM studies revealed synthesis of varied shaped AuNPs, viz., spheres, hexagons, triangles, rhomboids and rectangular etc. The presence of Au in the nanostructures was confirmed by EDS. The XRD pattern reflects the crystalline nature and nano size of AuNPs. These phage inspired AuNPs showed anti-bacterial activity against different bacterial pathogens. Anti-biofilm activity of AuNPs was evaluated on a glass slide. It was noticed that at 0.2 mM concentration of these AuNPs about 80% of biofilm formation by Pseudomonas aeruginosa, a human pathogen was inhibited. Thus, the phage inspired AuNPs synthesis could be potential therapeutic agents against human pathogens.