Abstract
BACKGROUND: Head and neck cancer (HNC) is the seventh most common malignant tumor. Herpesviruses are a significant risk factor in the multifactorial pathogenesis of HNC. OBJECTIVES: This study aimed to investigate the association between herpesviruses and the development of head and neck squamous cell carcinoma (HN-SCC). DESIGN: Experimental study. SETTING: A university hospital in Turkey. PATIENTS AND METHODS: Pathological archive tissue samples of 500 patients were included in the study. These samples were categorized into two groups: those diagnosed with HN-SCC (n=300, malignant group [MG]) and those diagnosed with benign head and neck lesions (n=200, benign group [BG]). The presence of herpesvirus in samples was detected using polymerase chain reaction. MAIN OUTCOME MEASURES: Association of herpesviruses in the development of head and neck cancer. SAMPLE SIZE: 500 patients. RESULTS: HHV-1, -2, -7, and -8 were not detected in any samples. In the malignant group (MG), EBV-DNA was detected in 1 patient (0.3%) and HHV-6 DNA in 2 patients (0.6%), while in the benign group (BG), VZV-DNA was detected in 1 patient (0.5%), EBV-DNA in 3 patients (1.5%), CMV-DNA in 5 patients (2.5%), and HHV-6 DNA in 3 patients (1.5%). While no significant difference was found between the groups for VZV, EBV, and HHV-6, a statistically significant difference was found in favor of the benign group for CMV. CONCLUSION: Although herpesvirus seroprevalence is relatively high in the population, the lack of viral genome in tissue samples indicates that other factors might be prominent in developing HN-SCC. LIMITATION: The storage conditions of the sample used (paraffinized sample) may have negatively affected the detection frequency of HHVs.