Abstract
Many studies focus on optimizing the dark fermentative biohydrogen production. In the present work, static magnetic field (SMF) S pole and N pole were applied to the dark fermentation system of Clostridium pasteurianum to evaluate the effect of magnetic field direction on biohydrogen production process of Clostridium pasteurianum. The experimental results showed that the magnetic S pole application was more effective than the magnetic N pole application in improving microbial hydrogen production progress parameters. The maximum hydrogen production 2.47 ± 0.010 mol H(2)/mol glucose was achieved in the magnetic S pole application group. Meanwhile, the maximum biomass, carbon conversion efficiency and energy conversion efficiency in magnetic S pole application group were increased by 93%, 27.7% and 28.3%, respectively, compared with the control group. The results provided a new information for further exploring the application of magnetic field in dark fermentative biohydrogen production process.