Physiochemical and bactericidal activity evaluation: Silver-augmented 3D-printed scaffolds-An in vitro study

物理化学和杀菌活性评估:银增强3D打印支架-体外研究

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作者:Vasudev Vivekanand Nayak, Nick Tovar, Jacques Henri Hacquebord, Simone Duarte, Beatriz H D Panariello, Caroline Tonon, Pablo J Atria, Paulo G Coelho, Lukasz Witek

Results

XRD, FTIR, ICP-MS, TGA, and DSC confirmed presence of Ag and PO4 groups, whereas electron microscopy showed a decrease in Ca and an increase in Ag ions, decreasing Ca/P ratio with increasing surfactant concentrations. PrestoBlue assays yielded an increase in fluorescence cell counts among experimental groups with lower concentrations of Ag characterized by their characteristic trapezoidal shape whereas cytotoxicity was observed at higher concentrations. Similar observations were made with alkaline phosphatase assays. Antimicrobial evaluation showed reduced colony-forming units (CFU) among all experimental groups when compared to 100% β-TCP. β-TCP scaffolds augmented with Ag ions facilitate antibacterial effects while promoting osteoblast adhesion and proliferation.

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