Effect of active fraction isolated from the leaf extract of Dregea volubilis [Linn.] Benth. on plasma glucose concentration and lipid profile in streptozotocin-induced diabetic rats

从卷叶山龙眼叶提取物中分离的活性成分对链脲佐菌素诱导糖尿病大鼠血浆葡萄糖浓度和血脂谱的影响

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Abstract

OBJECTIVE: The objective of the present study was to evaluate the effect of active fraction from Dregea volubilis [Linn.] Benth leaves on serum glucose and lipid profile in normal and diabetic rats. MATERIALS AND METHODS: Diabetes was induced by streptozotocin in wistar rats. Petroleum ether, ethyl acetate, and ethanol extracts of Dregea volubilis [Linn.] Benth leaves were administered orally at a dose of 200 mg/kg, p.o. Metformin was used as standard Anti-diabetic drug (50 mg/kg, p.o). The extract showing for higher Anti-diabetic activity was subjected to column chromatography that led to isolation of an active fraction, which was given trivial name Dv-1. Dv-1 (100 mg/kg, p.o.) was studied for its hypoglycemic and hypolipidemic potential. RESULTS: Ethanol extract was found to lower the Fasting blood glucose (FBG) level significantly (p < 0.05) in diabetic rats. Dv-1 caused a significant (p < 0.05) reduction in FBG level. Additionally it also caused reduction in cholesterol, triglyceride levels and improvement in the HDL level in diabetic rats. CONCLUSION: Reduction in the FBG, cholesterol, triglyceride levels and improvement in the HDL by Dv-1 indicates that Dv-1 has Anti-diabetic activity along with anti hyperlipidemic efficacy and provides a scientific rationale for the use as an Anti-diabetic agent.

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