Abstract
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that affects members of the Cervidae family, including deer (Odocoileus spp.), elk (Cervus Canadensis spp.), and moose (Alces alces spp.). While CWD is a neurodegenerative disease, lymphoid accumulation of the abnormal isoform of the prion protein (PrP(Sc)) is detectable early in the course of infection. It has been shown that a large portion of the PrP(Sc) lymphoid accumulation in infected mule deer takes place on the surface of follicular dendritic cells (FDCs). In mice, FDC expression of PrP(C) has been shown to be essential for PrP(Sc) accumulation. FDCs have been shown to normally express high levels of PrP(C) in mice and humans but this has not been examined in natural hosts for CWD. We used double immunofluorescent labeling and confocal microscopy to determine the PrP(C) expression characteristics of B and T lymphocytes as well as FDCs in palatine tonsils of CWD-negative mule deer and elk. We detected substantial PrP(C) colocalization with all cellular phenotypic markers used in this study, not just with FDC phenotypic markers.