Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus

使用无整合仙台病毒从患有 X 连锁成骨不全症的泰国患者的真皮成纤维细胞中生成两种人类 iPSC 系(MDCUi001-A 和 MDCUi001-B)

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作者:Siraprapa Tongkobpetch, Ruttachuk Rungsiwiwut, Kamthorn Pruksananonda, Kanya Suphapeetiporn, Vorasuk Shotelersuk

Abstract

Two clones of human induced pluripotent stem cells (iPSCs) were generated from dermal fibroblasts isolated from a one-year-old Thai patient with X-linked osteogenesis imperfecta. The patient harbored a mutation, p.N459S, in the MBTPS2 gene. The cells were reprogrammed using an integration-free Sendai virus containing KLF4, c-MYC, OCT4 and SOX2. Both of the established iPSC lines (MDCUi001-A and MDCUi001-B) maintained normal karyotype, expressed pluripotent markers and differentiated into all three germ layers.

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