Results
BINGO can isolate up to 10 fluorophores from spectral imaging data for a single excitation. nine-color living HeLa cells were visualized distinctly with BINGO. It provides an important algorithmic tool for multiplex imaging studies, especially in intravital imaging. BINGO shows great potential in multicolor imaging for biomedical sciences. Availability and implementation: The source code used for this paper is available with the test data at https://github.com/Xinyuan555/BINGO_unmixing.