Association between quantitative bacterial culture of bronchoalveolar lavage fluid and antibiotic requirement in dogs with lower respiratory tract signs

支气管肺泡灌洗液定量细菌培养与犬下呼吸道症状患者抗生素需求之间的关联

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Abstract

BACKGROUND: Historically, positive bacterial cultures from the lower respiratory tract (LRT) have been considered clinically relevant when quantitative bacterial cultures of bronchoalveolar lavage fluid (BALF) were >1700 colony forming units (cfu)/mL. However, this threshold might not accurately predict a requirement for antibiotics. OBJECTIVES: To study whether quantitative BALF bacterial culture results were predictive of antibiotic requirement in dogs with LRT signs. ANIMALS: Thirty-three client-owned dogs. METHODS: Cross-sectional study. Dogs with positive quantitative bacterial culture of BALF were included. Dogs were divided into 2 groups, depending on whether they had a LRT infection requiring antibiotics (LRTI-RA) or LRT disease not requiring antibiotics (LRTD-NRA), based on thoracic imaging features, presence of intracellular bacteria on BALF cytology, and response to treatment. Predictive effect of cfu/mL and BALF total nucleated cell count (TNCC) on antibiotic requirement, adjusting for ongoing or prior antibiotic therapy and age, were studied using logistic regression. RESULTS: Twenty-two and 11 dogs were included in the LRTI-RA and LRTD-NRA groups, respectively. The cfu/mL was not significantly predictive of antibiotic requirement, independent of ongoing or prior antibiotic treatment and age (LRTI-RA: median, 10 000 cfu/mL; range, 10-3 × 10(8) ; LRTD-NRA: median, 10  000 cfu/mL; range, 250-1.3 × 10(9) ; P = .27). The TNCC was not significantly predictive of antibiotic requirement when only dogs with bronchial disease were considered (LRTI-RA: median, 470 cells/μL; range, 240-2260; LRTD-NRA: median, 455 cells/μL; range, 80-4990; P = .57). CONCLUSION AND CLINICAL IMPORTANCE: The cfu/mL is an inappropriate measure for determining whether antibiotics are of benefit in dogs with LRT signs.

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