Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and clustering of Neisseria gonorrhoeae

基质辅助激光解吸电离飞行时间质谱 (MALDI-TOF MS) 对淋病奈瑟菌的鉴定和聚类的评估

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作者:Anna Carannante, Elena De Carolis, Paola Vacca, Antonietta Vella, Caterina Vocale, Maria Antonia De Francesco, Marco Cusini, Simonetta Del Re, Ivano Dal Conte, Antonio Cristaudo, Patrizia Ober, Maurizio Sanguinetti, Paola Stefanelli

Background

The sexually transmitted infection gonorrhea remains a public health concern for becoming resistant to drug treatments available. The

Conclusion

MALDI-TOF MS for a real-time detection and cluster analysis of gonorrhea is a promising tool for surveillance purposes. Moreover, additional studies are required to collect more data on the performance of MALDI-TOF MS for gonococci.

Results

Molecular typing by NG-MAST showed 28 sequence types (STs); G1407 was the predominant accounting for 75 gonococci. All the 93 gonococci, except one, were correctly identified at species level by MALDI-TOF MS and G1407 isolates were divided into two clusters.

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