Abstract
The development of bioactive dental materials with antimicrobial and biocompatible properties is important for improving clinical outcomes and reducing complications associated with intraoral devices. This study presents a novel approach that combines a 3D-printed shape-memory resin (TC-85DAC) with green-synthesized zinc oxide nanoparticles (ZnO NPs) to enhance biological performance. ZnO NPs were synthesized using Dysphania ambrosioides extract, producing quasi-spherical particles with a crystalline hexagonal structure and sizes between 15 and 40 nm. Resin discs were coated with ZnO NPs at 10%, 20%, and 30%, then assessed for biocompatibility with human gingival fibroblasts and antibacterial activity against Porphyromonas gingivalis and Streptococcus mutans. Surface roughness was also considered with and without ZnO NPs. Biocompatibility assays revealed a concentration- and time-dependent increase in cell viability, with the highest values at 30% ZnO NPs after 72 h of exposure to the NPs. Antibacterial testing confirmed the inhibition of both species, with Porphyromonas gingivalis showing greater sensitivity. Surface roughness increased with higher ZnO NPs concentrations, significantly influencing biological interactions. The integration of green-synthesized ZnO NPs with shape-memory resin produced a multifunctional dental material with improved bioactivity. This sustainable strategy enables bioactive coatings on 3D-printed resins, with potential applications in the next generation of smart dental devices.