Automatic DNA replication tract measurement to assess replication and repair dynamics at the single-molecule level

自动 DNA 复制道测量,以评估单分子水平上的复制和修复动力学

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作者:Longjie Li, Arun Mouli Kolinjivadi, Kok Haur Ong, David M Young, Gabriel Pik Liang Marini, Sock Hoai Chan, Siao Ting Chong, Ee Ling Chew, Haoda Lu, Laurent Gole, Weimiao Yu, Joanne Ngeow

Results

To address the unmet needs of diverse DNA fibre analysis investigations, we propose DNA Stranding, an open-source software that is able to perform accurate fibre length quantification (13.22% mean relative error) and fibre pattern recognition (R > 0.93) with up to six fibre patterns supported. With the graphical interface, we developed, user can conduct semi-automatic analyses which benefits from the advantages of both automatic and manual processes to improve workflow efficiency without compromising accuracy. Availability and implementation: The software package is available at https://github.com/lgole/DNAStranding.

Supplementary Information

Supplementary data are available at Bioinformatics online.

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