Abstract
Magnetotactic bacteria (MTB), a unique group of Gram-negative prokaryotes, have the remarkable ability to biomineralize magnetic nanoparticles (MNPs) intracellularly, making them promising candidates for various biomedical applications such as biosensors, drug delivery, imaging contrast agents, and cancer-targeted therapies. To fully exploit the potential of MTB, a precise understanding of the structural, surface, and functional properties of these biologically produced nanoparticles is required. Given these concerns, this review provides a focused synthesis of the most widely used microscopic and spectroscopic methods applied in the characterization of MTB and their associated MNPs, covering the latest research from January 2022 to May 2025. Specifically, various optical microscopy techniques (e.g., transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM)) and spectroscopic approaches (e.g., localized surface plasmon resonance (LSPR), surface-enhanced Raman scattering (SERS), and X-ray photoelectron spectroscopy (XPS)) relevant to ultrasensitive MTB biosensor development are herein discussed and compared in term of their advantages and disadvantages. Overall, the novelty of this work lies in its clarity and structure, aiming to consolidate and simplify access to the most current and effective characterization techniques. Furthermore, several gaps in the characterization methods of MTB were identified, and new directions of methods that can be integrated into the study, analysis, and characterization of these bacteria are suggested in exhaustive manner. Finally, to the authors' knowledge, this is the first comprehensive overview of characterization techniques that could serve as a practical resource for both younger and more experienced researchers seeking to optimize the use of MTB in the development of advanced biosensing systems and other biomedical tools.