Background
Human adipose-derived stem cells (hADSCs) are capable of differentiating into many cells such as cardiac cells. Different types of inducers are used for cardiac cell differentiation, but this question still remains to be investigated, which one is the best. The
Conclusions
We concluded that the TSA alone or with fibrin scaffold can lead to the generation of cardiac like cells in a short period of time.
Methods
After approval of characteristics of hADSCs and fibrin scaffold, hADSCs were cultured in fibrin scaffold with 10 µM TSA for 72 h and kept in standard conditions for 4 weeks. QRT-PCR and immunostaining assay were performed for evaluating the expression pattern of special cardiac genes and proteins.
Results
In particular, our study showed that fibrin scaffold alongside TSA enhanced expression of the selected genes and proteins. Conclusions: We concluded that the TSA alone or with fibrin scaffold can lead to the generation of cardiac like cells in a short period of time.