Abstract
BACKGROUND: In the opinion of all field herpetologists and archaeozoologists, green frogs (living and sub-fossil specimens)are among the most difficult European amphibians to identify. Here, we examine the intra- and interspecific variation in both continuous and discrete iliac characters for refining the criteria for species-level identification among Europeangreen frogs Pelophylaxridibundus,Pelophylaxlessonae andtheir associated klepton Pelophylaxkl.P.esculentus (Amphibia,Ranidae). RESULTS: Atotal of 454 ilia of modern green frogs from the former German Democratic Republic have been studied: 168 for P.lessonae (86of males and 82 of females), 118 for P.ridibundus (44of males and 74 of females) and 168 for P.kl.P.esculentus (86of males and 82 of females). The origin, sex, population structure and phenotype are known for each of the studied specimens. Eight variables have been taken (one angle and seven measurements), mainly on the distal part of the element in order to be able to apply them to fragmentary fossil ilia. Interspecific variations, sexual dimorphism and population structure have been investigated. Results suggest that the secure determination of a single fossil ilium at species level is quite impossible, but that at population level, it may be possible to distinguish between a 'pure' species or a 'pure' species plus its cohabiting hybrid form, as some minor differences have been evidencedin particular in the angle of the tubersuperior inrelation to iliac's main axis (character 2) and the width of the parsascendens (character8); two parameters significantly non size-dependent. No sexual dimorphism has been detected, except for P.lessonae. CONCLUSIONS: We propose here a new biometrical method in order to differentiate between the fossil ilia of central European water frogs (genus Pelophylax) at population level.