Measurement of intestinal permeability using lactulose and mannitol with conventional five hours and shortened two hours urine collection by two different methods: HPAE-PAD and LC-MSMS

使用乳果糖和甘露醇通过两种不同的方法测量肠道通透性,包括常规五小时和缩短两小时尿液收集:HPAE-PAD 和 LC-MSMS

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作者:Md Abu Musa, Mamun Kabir, Md Iqbal Hossain, Emtiaz Ahmed, Abdullah Siddique, Humaira Rashid, Mustafa Mahfuz, Dinesh Mondal, Tahmeed Ahmed, William A Petri, Rashidul Haque

Methods

HPAE-PAD and LC-MSMS. The lactulose: mannitol (LM) test was performed in a cohort of Bangladeshi infants considered at-risk for environmental enteropathy. 208 urine specimens from 104 (52 male and 52 female) infants were collected at 2 and 5 hours after LM solution administration and were tested for lactulose and mannitol by two different methods, one HPAE-PAD platform and another LC-MSMS platform. Median age of the children was 15.0 months (range 6.9 to 25.8 months) and their mean weight-for-age z-score was -0.92. A higher percentage of lactulose and mannitol recovery was found in 5 hours urine collection than in the corresponding 2 hours by both HPAE-PAD and LC-MSMS method, but when

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