Abstract
Axon pruning facilitates the removal of ectopic and misguided axons and plays an important role in neural circuit formation during brain development. Sema3F and its receptor neuropilin-2 (Nrp2) have been shown to be involved in the stereotyped pruning of the infrapyramidal bundle (IPB) of mossy fibers of the dentate gyrus (DG) in the developing hippocampus. Collapsin response mediator proteins (CRMPs) were originally identified as an intracellular mediator of semaphorin signaling, and the defective pruning of IPB was recently reported in CRMP2-/- and CRMP3-/- mice. CRMP1 and CRMP4 have high homology to CRMP2 and CRMP3, and their expression in the developing mouse brain overlaps; however, their role in IPB pruning has not yet been examined. In this study, we report that CRMP4, but not CRMP1, is involved in IPB pruning during neural circuit formation in the hippocampus. Our genetic interaction analyses indicated that CRMP2 and CRMP4 have distinct functions and that CRMP2 mediates IPB pruning via Nrp2. We also observed the altered synaptic terminals of mossy fibers in CRMP2 and CRMP4 mutant mice. These results suggest that CRMP family members have a distinct function in the axon pruning and targeting of mossy fibers of the hippocampal DG in the developing mouse brain.