Abstract
Biochemical characterization of new microalgal strains that are isolated from diverse environmental conditions is an important starting point for the establishment of high-quality feedstock for nutraceutical and pharmaceutical applications. In this research study, the biochemical composition of three Iranian native subspecies of Scenedesmus microalgae (Scenedesmus obliquus, Scenedesmus bijugusi, and Scenedesmus sp.), with the main focus on fatty acid composition, was studied. The results showed that the strain Scenedesmus bijugusi had the highest biomass productivity (48 g/L/d), biomass (0.73%), carbohydrate (13.97%), fat (16.27%), protein (44.04%), chlorophyll-a (6.32 mg/g), and carotenoids (3.7 mg/g). The lipid profile also revealed that S. obliquus had the highest percentage of polyunsaturated fatty acid (46.52%), ratio of ∑n-3/∑n-6 (5.96), ratio of polyunsaturated fatty acid to saturated fatty acid (PUFA/SAF) (1.18), α-linolenic acid (22.74%), hypocholesterolemia index (1.61), and low atherogenic index (0.34). S. bijugusi and S. obliquus, thus, showed a great promise in nutraceutical and pharmaceutical applications due to their appropriate high productivity, biopigment, protein, lipid, antioxidant activity, long-chain polyunsaturated fatty acids, and α-linolenic acid.