Abstract
The production of spicy sauces from chili peppers (Capsicum spp.) generates 5-30% of spicy by-product which is rich in valuable compounds (e.g., capsaicinoids, carotenoids, phenolics, etc.) and can serve as a source of Capsicum oleoresins, providing spice and color ingredients for food products. This study primarily focused on the optimization of Capsicum oleoresin extraction from Red Habanero chili pepper (Capsicum chinense Jacq.) by-product using ultrasound-assisted extraction (UAE). A second focus was the comparison between UAE and reflux-assisted extraction (RAE). Response Surface Methodology (RSM) was employed to optimize the extraction time (3 to 17 min) and acoustic power density (APD, 0.30 to 1.00 W/mL). The optimal UAE conditions (8 min, 0.87 W/mL) showed a higher extraction yield (26%) and high quality oleoresin extracts rich in bioactives (capsaicinoids: 7 mg/g; phenolics: 4 mg GAE/g) with antioxidant activity (FRAP: 139 µmol FeSO(4) eq/g; DPPH: 33 µmol TEAC/g). Optimum UAE extracts proved more colored, energy-efficient (95% less consumption), equally spicy (466,000 SHU) and had higher antioxidant activity than RAE. These results demonstrated UAE as a sustainable method for producing high value spicy additives from chili pepper by-product, turning them into products with enhanced bioactivity, favoring a circular economy in the agri-food industry.