Abstract
The interaction effects of organic ligand ethylene diamine tetra-acetic acid (EDTA) and oxide nanoparticles (magnetite Fe(3)O(4)-NPs and copper CuO-NPs) were investigated during a 72 h period on two green algal species-Chlamydomonas reinhardtii under freshwater conditions and Chlamydomonas euryale under saltwater conditions. Fe(3)O(4)-NPs had larger agglomerates and very low solubility. CuO-NPs, having smaller agglomerates and higher solubility, were more toxic than Fe(3)O(4)-NPs in freshwater conditions for similar mass-based concentrations, especially at 72 h under 100 mg L(-1). Furthermore, the effect of EDTA increased nanoparticle solubility, and the salinity caused a decrease in their solubility. Our results on C. euryale showed that the increase in salinity to 32 g L(-1) caused the formation of larger nanoparticle agglomerates, leading to a decrease in the toxicity impact on algal cells. In addition, EDTA treatments induced a toxicity effect on both freshwater and saltwater Chlamydomonas species, by altering the nutrient uptake of algal cells. However, C. euryale was more resistant to EDTA toxicity than C. reinhardtii. Moreover, nanoparticle treatments caused a reduction in EDTA toxicity, especially for CuO-NPs. Therefore, the toxicity impact caused by these environmental factors should be considered in risk assessment for metallic nanoparticles.