Abstract
Asynchronous flowering between male and female date palms (Phoenix dactylifera L.) makes pollen storage a practical necessity for growers, especially for cultivars like 'Mejhoul', which require artificial pollination. This study examined the stainability of pollen as an indicator of cytoplasmic integrity, from four male date plant pollen donor genotypes ('Mejhoul', 'Deglet Nour', 'Khadrawy', and 'Zahidi') stored at 4 °C for different durations (fresh, one-year, and two-year storage) and their effects on fruit set and physical fruit characteristics of the Mejhoul cultivar in Mexico. Pollen stainability was assessed in vitro using 1% acetocarmine. Fruit and seed set percentages were evaluated as indicators of the practical effectiveness of stored pollen under field conditions, but not as direct measures of viability. Results showed that fresh pollen exhibited the highest stainability (91.2-95.6%), followed by one-year-stored pollen (59.4-68.3%), and two-year-stored pollen (38.8-45.4%). Fruit set percentages were highest with fresh pollen (63.8-81.7%), decreasing with storage duration. 'Deglet Nour' pollen consistently showed superior compatibility with 'Mejhoul' females. Physical fruit characteristics (weight, length, diameter) and seed traits were minimally affected by reduced pollen stainability, indicating that there were enough viable grains for effective pollination. The study also observed Metaxenia and Xenia effects, where pollen genotypes influenced fruit and seed size. Overall, these findings suggest that pollen stored at 4 °C for short and medium terms can be used in Mejhoul production, but longer storage significantly reduces efficacy, recommending sub-zero temperatures for extended preservation.