Characterization of a potential probiotic bacterium Lactococcus raffinolactis WiKim0068 isolated from fermented vegetable using genomic and in vitro analyses

使用基因组和体外分析对从发酵蔬菜中分离的潜在益生菌 Lactococcus raffinolactis WiKim0068 进行表征

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作者:Min Young Jung, Changsu Lee, Myung-Ji Seo, Seong Woon Roh, Se Hee Lee

Background

Lactococcus members belonging to lactic acid bacteria are widely used as starter bacteria in the production of fermented dairy products. From kimchi, a Korean food made of fermented vegetables, Lactococcus raffinolactis WiKim0068 was isolated and its genome was analyzed.

Conclusion

These results augments our understanding of the genus Lactococcus and suggest that this new strain has potential industrial applications.

Results

The complete genome of the strain WiKim0068 consists of one chromosome and two plasmids that comprises 2,292,235 bp, with a G + C content of 39.7 mol%. Analysis of orthoANI values among Lactococcus genome sequences showed that the strain WiKim0068 has > 67% sequence similarity to other species and subspecies. In addition, it displayed no antibiotic resistance and can metabolize nicotinate and nicotinamide (vitamin B3).

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