Abstract
The world urgently needs new methods to quickly and efficiently detect mutated viruses. An RNA-AuNP-based colorimetric biosensor is a highly sensitive, specific, and cost-effective tool that enables rapid, visual detection of target molecules for applications in disease diagnostics, environmental monitoring, and forensic analysis. An RNA-AuNP-based colorimetric biosensor requires precise control over nanoparticle dispersion and aggregation, which can be achieved using temperature regulation. A novel on-chip microelectrode is proposed to induce and monitor the aggregation of RNA-attached gold nanoparticles (AuNPs) through Joule heating and impedance spectroscopy. The proposed platform is implemented based on printed circuit board (PCB) technology, which has many advantages, such as fast and easy design and fabrication, low power consumption, and low costs. Joule heating is the process in which the energy of an electric current is converted into heat as it flows through a resistance. Impedance spectroscopy is an analytical technique that measures a system's electrical response to an applied AC signal across a range of frequencies, providing insights into a sample's dielectric properties. The results validate that the fabricated microelectrode is capable of heating a 20 µL droplet to 75 °C within 30 s, utilizing a low power input of only 3.75 watts and successfully inducing a color change based on the presence of hepatitis C virus (HCV) RNA, while impedance readings are used to monitor the aggregation.