Abstract
BACKGROUND: Peste des petits ruminants virus (PPRV) is one of the most economically important pathogens in sheep and goats. Fusion (F) and Hemagglutinin (H) proteins are the main immune-stimulating antigens. AIMS: The present study aimed to clone and express F and H genes in baculovirus, and evaluate the immunogenicity of recombinant proteins produced by sf9 cells in mice. METHODS: Amplified F and H genes (by RT-PCR using specific primers) were cloned into pFastBac Dual plasmid. The recombinant plasmid was transformed in DH10Bac host cells. SDS-PAGE and Western blotting was performed to control the recombinant protein, and a whole pure and specific recombinant protein was obtained. RESULTS: The immunogenicity of 20 μg of non-adjuvant recombinant proteins in Balb-c mice showed better results compared with the attenuated PPR vaccine. CONCLUSION: The recombinant protein obtained from this study can be a suitable candidate for the production of recombinant vaccines against PPRV.