Abstract
PURPOSE: External electric fields can regulate the neural network and change the excitability of the in-vivo cerebral cortex. Here, to prove the effect of alternating electric fields on the synaptic plasticity of ex-vivo tissues, the regular changes in the synaptic structure under alternating electric fields were studied. METHODS: This study applied alternating electric fields with a peak voltage of 20 V and frequencies of 5, 20, 50, and 80 Hz to the porcine cerebral cortex. Relying on transmission electron microscopy (TEM), the ultrastructure of synapses was observed, and the curvature radius of post-synaptic density (PSD) and the synaptic gap distance was quantified. RESULTS: The results indicated that under alternating electric fields, the average synaptic curvature of the PSD decreased by 30-59% with increasing frequency, and the average synaptic gap distance became narrower. CONCLUSION: In ex-vivo brain tissue, synaptic plasticity can be regulated by alternating electric fields of different frequencies. This study can provide reference data for the storage and regulation of ex-vivo organs, as well as comparable data for in-vivo studies.