Methods to Identify Rat and Mouse Retinal Ganglion Cells in Retinal Flat-Mounts

识别大鼠和小鼠视网膜平板中的视网膜神经节细胞的方法

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作者:Juan A Miralles de Imperial-Ollero, Beatriz Vidal-Villegas, Alejandro Gallego-Ortega, Francisco M Nadal-Nicolás, Manuel Salinas-Navarro, María Norte-Muñoz, Johnny Di Pierdomenico, Caridad Galindo-Romero, Marta Agudo-Barriuso, Manuel Vidal-Sanz, Francisco J Valiente-Soriano

Abstract

The identification of distinct retinal ganglion cell (RGC) populations in flat-mounted retinas is key to investigating pathological or pharmacological effects in these cells. In this chapter, we review the main techniques for detecting the total population of RGCs and various of their subtypes in whole-mounted retinas of pigmented and albino rats and mice, four of the animal strains most studied by the scientific community in the retina field. These methods are based on the studies published by the Vidal-Sanz's laboratory.

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