Abstract
Lei Chen works in the field of clinical diagnosis of antifungal tolerance and persistence. In this mSphere of Influence, he reflects on how the papers "Antifungal tolerance is a subpopulation effect distinct from resistance and is associated with persistent candidemia" by A. Rosenberg, I. V. Ene, M. Bibi, S. Zakin, et al. (Nat Commun 9:2470, 2018, https://doi.org/10.1038/s41467-018-04926-x) and "Macrophage internalization creates a multidrug-tolerant fungal persister reservoir and facilitates the emergence of drug resistance" by A. Arastehfar, F. Daneshnia, N. Cabrera, S. Penalva-Lopez, et al. (Nat Commun 14:1183, 2023, https://doi.org/10.1038/s41467-023-36882-6) impacted his work on the development of diagnostic methods for antifungal tolerance and persistence.