Enhancing genotyping of MAOA-LPR and 5-HTT-LPR in rhesus macaques (Macaca mulatta)

提高恒河猴(Macaca mulatta)MAOA-LPR 和 5-HTT-LPR 的基因分型能力

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Abstract

BACKGROUND: Genetic variation in monoamine oxidase A (MAOA) and serotonin transporter (5-HTT)-linked polymorphic regions (LPR) is associated with neuropsychiatric behavior. METHODS: We genotyped 37 macaques using conventional PCR product gel fractionation and by capillary electrophoresis of multiplexed amplicons and compared the data. RESULTS: Genotype concordance was 97% and 95% for MAOA-LPR and 5-HTT-LPR, respectively. Capillary electrophoresis was more sensitive and cost-effective. CONCLUSIONS: Multiplexing MAOA-LPR and 5-HTT-LPR will enhance the genotyping of large sample sets.

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