Abstract
The aim of this study was to compare the effects of electrolytic and nonelectrolytic toothbrushing on dogs' oral health and the presence of common bacteria associated with periodontal disease. Periodontal disease in dogs is a common problem worldwide. A toothbrushing procedure is recommended to prevent periodontal disease, with additional benefits if electrolytic toothbrushes are used in dog oral hygiene practices. A total of 26 dogs were enrolled in this eight-week study and were divided into two groups-treatment and control. Daily toothbrushing was performed on all dogs using the same dog toothbrush, with the power source disengaged in the control group. Oral examination was conducted on anesthetized dogs before and at 4 and 8 weeks after commencing the study, with sampling for bacterial analysis. This study was designed to be blind for owners, veterinarians, and laboratory staff. Improvements in the average gingival index (from 0.55 to 0.31) and calculus index (from 0.55 to 0.38) in the treatment group were recorded. In the control group, after an initial improvement in the plaque index (from 0.97 to 0.53), at week 8, it significantly rose to 1.21 (p < 0.05). Relative bacterial abundance revealed a reduction in all four tested bacteria in the treatment group, while in the control group, Campylobacter rectus levels rose by 3.67 log2 compared to before the study and at week 8. No adverse effects were recorded in either group.