Abstract
Insect-pollinated plants are essential for honey bees to feed their brood. In agricultural landscapes, honey bees and other pollinators are often exposed to pesticides used for cultivation. In order to gain more insight into the fluctuation of pesticide loads, 102 daily pollen samples were collected between April and July 2018 in a fruit-growing area in Southern Germany. Samples were analyzed with respect to more than 260 pesticides using a multi-residue pesticide analysis method. Almost 90% of the analyzed pollen samples featured between one and thirteen different pesticides. In total, 29 pesticides were detected at maximum concentrations of up to 4500 ng/g pollen. Maximum residual concentrations of most pesticides were observed during April and the first half of May, as well as during the second half of June. In most cases, serial data of pesticide residuals were detected for approximately 10 subsequent days with two or three maximum values, which were several folds higher than concentrations on the days before and thereafter. The pollen hazard quotient (PHQ) was calculated to estimate the risk of the detected pesticides to honey bees and wild pollinators.