Abstract
Time domain nuclear magnetic resonance (TD-NMR) has been widely applied in plant science in the last four decades. Several TD-NMR instruments and methods have been developed for laboratory, green-house, and field studies. This mini-review focuses on the recent TD-NMR pulse sequences applied in plant science. One of the sequences measures the transverse relaxation time (T(2)) with minimal sample heating, using a lower refocusing flip angle and consequently lower specific absorption rate than that of conventional CPMG. Other sequences are based on a continuous wave free precession (CWFP) regime used to enhance the signal-to-noise ratio, to measure longitudinal (T(1)) and transverse relaxation time in a single shot experiment, and as alternative 2D pulse sequences to obtain T(1)-T(2) and diffusion-T(1) correlation maps. This review also presents some applications of these sequences in plant science.