Abstract
This study explores the floristic diversity of wild edible plants (WEPs) in the area surrounding Tirli, a small village in the Tuscan Maremma, Italy. Field surveys identified 128 vascular plant taxa across 46 families and 106 genera, with Asteraceae (26 taxa), Rosaceae (10 taxa), and Lamiaceae (8 taxa) being the most represented. The dominant life-forms are scapose Hemicryptophytes, scapose Therophytes, and rosulate Hemicryptophytes, with Euro-Mediterranean, Subcosmopolitan, and Steno-Mediterranean distributions prevailing. Statistical analyses revealed significant associations between life-forms and edible plant parts: scapose and rosulate Hemicryptophytes were linked to leaf use, scapose Therophytes to root use, and Phanerophytes to fruit use. The Asteraceae family exhibited exceptional versatility, being associated with various edible parts. Notably, the endemic species Centaurea nigrescens Willd. subsp. pinnatifida (Fiori) Dostál was recorded for the first time in the Tuscan Maremma, underscoring the area's naturalistic value. Traditional culinary practices were linked to some edible plants, which were analyzed for bioactive compounds, including photosynthetic pigments, primary metabolites, secondary metabolites, and antioxidant activity. The results confirmed their biochemical richness and functional properties. This study emphasizes the ecological, nutritional, and cultural significance of Tirli's wild edible flora, promoting biodiversity conservation, cultural heritage preservation, and sustainable food practices.