The sequence and de novo assembly of the giant panda genome

大熊猫基因组的序列测定和从头组装

阅读：1
作者：Li,Ruiqiang,Fan,Wei,Tian,Geng,Zhu,Hongmei,He,Lin,Cai,Jing,Huang,Quanfei,Cai,Qingle,Li,Bo,Bai,Yinqi,Zhang,Zhihe,Zhang,Yaping,Wang,Wen,Li,Jun,Wei,Fuwen,Li,Heng,Jian,Min,Li,Jianwen,Zhang,Zhaolei,Nielsen,Rasmus,Li,Dawei,Gu,Wanjun,Yang,Zhentao,Xuan,Zhaoling,Ryder,Oliver A,Leung,Frederick Chi-Ching,Zhou,Yan,Cao,Jianjun,Sun,Xiao,Fu,Yonggui,Fang,Xiaodong,Guo,Xiaosen,Wang,Bo,Hou,Rong,Shen,Fujun,Mu,Bo,Ni,Peixiang,Lin,Runmao,Qian,Wubin,Wang,Guodong,Yu,Chang,Nie,Wenhui,Wang,Jinhuan,Wu,Zhigang,Liang,Huiqing,Min,Jiumeng,Wu,Qi,Cheng,Shifeng,Ruan,Jue,Wang,Mingwei,Shi,Zhongbin,Wen,Ming,Liu,Binghang,Ren,Xiaoli,Zheng,Huisong,Dong,Dong,Cook,Kathleen,Shan,Gao,Zhang,Hao,Kosiol,Carolin,Xie,Xueying,Lu,Zuhong,Zheng,Hancheng,Li,Yingrui,Steiner,Cynthia C,Lam,Tommy Tsan-Yuk,Lin,Siyuan,Zhang,Qinghui,Li,Guoqing,Tian,Jing,Gong,Timing,Liu,Hongde,Zhang,Dejin,Fang,Lin,Ye,Chen,Zhang,Juanbin,Hu,Wenbo,Xu,Anlong,Ren,Yuanyuan,Zhang,Guojie,Bruford,Michael W,Li,Qibin,Ma,Lijia,Guo,Yiran,An,Na,Hu,Yujie,Zheng,Yang,Shi,Yongyong,Li,Zhiqiang,Liu,Qing,Chen,Yanling,Zhao,Jing,Qu,Ning,Zhao,Shancen,Tian,Feng,Wang,Xiaoling,Wang,Haiyin,Xu,Lizhi,Liu,Xiao,Vinar,Tomas,Wang,Yajun,Lam,Tak-Wah,Yiu,Siu-Ming,Liu,Shiping,Zhang,Hemin,Li,Desheng,Huang,Yan,Wang,Xia,Yang,Guohua,Jiang,Zhi,Wang,Junyi,Qin,Nan,Li,Li,Li,Jingxiang,Bolund,Lars,Kristiansen,Karsten,Wong,Gane Ka-Shu,Olson,Maynard,Zhang,Xiuqing,Li,Songgang,Yang,Huanming,Wang,Jian,Wang,Jun
期刊：Nature影响因子：48.500
时间：2010起止号：2010 Jan 21;463(7279):311-7
doi：10.1038/nature08696

Abstract

Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes.

特别声明

1、本页面内容包含部分的内容是基于公开信息的合理引用；引用内容仅为补充信息，不代表本站立场。

2、若认为本页面引用内容涉及侵权，请及时与本站联系，我们将第一时间处理。

3、其他媒体/个人如需使用本页面原创内容，需注明“来源：[生知库]”并获得授权；使用引用内容的，需自行联系原作者获得许可。

4、投稿及合作请联系：info@biocloudy.com。