Abstract
Fluorescence molecular tomography (FMT) is a novel imaging modality to obtain fluorescence biomarkers' three-dimensional (3D) distribution. However, the simplified mathematical model and complicated inverse problem limit it to achieving precise results. In this study, the second near-infrared (NIR-II) fluorescence imaging was adopted to mitigate tissue scattering and reduce noise interference. An excitation-based fully connected network was proposed to model the inverse process of NIR-II photon propagation and directly obtain the 3D distribution of the light source. An excitation block was embedded in the network allowing it to autonomously pay more attention to neurons related to the light source. The barycenter error was added to the loss function to improve the localization accuracy of the light source. Both numerical simulation and in vivo experiments showed the superiority of the novel NIR-II FMT reconstruction strategy over the baseline methods. This strategy was expected to facilitate the application of machine learning in biomedical research.